SuicidDentistV1, Main, Exploration, bibRecord, 000156

Effects of human vascular endothelial growth factor on reparative dentin formation.

Identifieur interne : 000156 ( Main/Exploration ); précédent : 000155; suivant : 000157

Effects of human vascular endothelial growth factor on reparative dentin formation.

Auteurs : Juan Zhang [République populaire de Chine] ; Xia Liu [République populaire de Chine] ; Weixian Yu [République populaire de Chine] ; Yingli Zhang [République populaire de Chine] ; Ce Shi [République populaire de Chine] ; Shilei Ni [République populaire de Chine] ; Qilin Liu [République populaire de Chine] ; Xiangwei Li [République populaire de Chine] ; Yingjian Sun [République populaire de Chine] ; Changyu Zheng [États-Unis] ; Hongchen Sun [République populaire de Chine]

Source :

Molecular medicine reports [ 1791-3004 ] ; 2016.

RBID : pubmed:26647730

Descripteurs français

KwdFr :
- Analyse de profil d'expression de gènes (MeSH), Animaux (MeSH), Calcification physiologique (effets des médicaments et des substances chimiques), Cicatrisation de plaie (effets des médicaments et des substances chimiques), Dent de lait (cytologie), Dentine (métabolisme), Différenciation cellulaire (effets des médicaments et des substances chimiques), Enfant (MeSH), Facteur de croissance endothéliale vasculaire de type A (pharmacologie), Humains (MeSH), Mâle (MeSH), Pulpe dentaire (cytologie), Rat Wistar (MeSH), Régulation de l'expression des gènes (effets des médicaments et des substances chimiques).
MESH :
- cytologie : Dent de lait, Pulpe dentaire.
- effets des médicaments et des substances chimiques : Calcification physiologique, Cicatrisation de plaie, Différenciation cellulaire, Régulation de l'expression des gènes.
- métabolisme : Dentine.
- pharmacologie : Facteur de croissance endothéliale vasculaire de type A.
- Analyse de profil d'expression de gènes, Animaux, Enfant, Humains, Mâle, Rat Wistar.

English descriptors

KwdEn :
- Animals (MeSH), Calcification, Physiologic (drug effects), Cell Differentiation (drug effects), Child (MeSH), Dental Pulp (cytology), Dentin (metabolism), Gene Expression Profiling (MeSH), Gene Expression Regulation (drug effects), Humans (MeSH), Male (MeSH), Rats, Wistar (MeSH), Tooth, Deciduous (cytology), Vascular Endothelial Growth Factor A (pharmacology), Wound Healing (drug effects).
MESH :
- chemical , pharmacology : Vascular Endothelial Growth Factor A.
- cytology : Dental Pulp, Tooth, Deciduous.
- drug effects : Calcification, Physiologic, Cell Differentiation, Gene Expression Regulation, Wound Healing.
- metabolism : Dentin.
- Animals, Child, Gene Expression Profiling, Humans, Male, Rats, Wistar.

Abstract

It is a challenge for dentists to save dental pulp in patients with pulp disease without resorting to root canal therapy. Formation of tertiary dentin to maintain pulp vitality is a key odontoblast response to dental pulp injury. Vascular endothelial growth factor (VEGF) is the most potent angiogenic and vasculogenic factor involved in tertiary dentin formation. It was hypothesized that VEGF may be used to treat pulp diseases such as pulpitis. To explore this hypothesis, the first step was to assess whether VEGF affects dental pulp cells to promote reparative dentin formation. In the current study, an AdCMV‑hVEGF vector was constructed to deliver hVEGF into dental pulp cells of exfoliated deciduous teeth (hDPCs) in vitro and dental pulp cells in a rat model in vivo. The collected data clearly demonstrated that hVEGF increased alkaline phosphatase and mineralization by enzymatic activity. RT‑qPCR data demonstrated that hVEGF significantly increased the expression levels of genes commonly involved in osteogenesis/odontogenesis. Data from the in vivo assays indicated that hVEGF enhanced pulp cell proliferation and neovascularization, and markedly increased formation of reparative dentin in dental pulp. The in vitro and in vivo data suggest that hVEGF may have potential clinical applications, thus may aid in the development of novel treatment strategies for dental pulpitis.

DOI: 10.3892/mmr.2015.4608
PubMed: 26647730
PubMed Central: PMC4686068

Affiliations:

Links toward previous steps (curation, corpus...)

to stream Main, to step Corpus: 000162
to stream Main, to step Curation: 000162

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Effects of human vascular endothelial growth factor on reparative dentin formation.</title>
<author><name sortKey="Zhang, Juan" sort="Zhang, Juan" uniqKey="Zhang J" first="Juan" last="Zhang">Juan Zhang</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Xia" sort="Liu, Xia" uniqKey="Liu X" first="Xia" last="Liu">Xia Liu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yu, Weixian" sort="Yu, Weixian" uniqKey="Yu W" first="Weixian" last="Yu">Weixian Yu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Yingli" sort="Zhang, Yingli" uniqKey="Zhang Y" first="Yingli" last="Zhang">Yingli Zhang</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shi, Ce" sort="Shi, Ce" uniqKey="Shi C" first="Ce" last="Shi">Ce Shi</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ni, Shilei" sort="Ni, Shilei" uniqKey="Ni S" first="Shilei" last="Ni">Shilei Ni</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Qilin" sort="Liu, Qilin" uniqKey="Liu Q" first="Qilin" last="Liu">Qilin Liu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Xiangwei" sort="Li, Xiangwei" uniqKey="Li X" first="Xiangwei" last="Li">Xiangwei Li</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Yingjian" sort="Sun, Yingjian" uniqKey="Sun Y" first="Yingjian" last="Sun">Yingjian Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130021</wicri:regionArea>
<wicri:noRegion>Jilin 130021</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zheng, Changyu" sort="Zheng, Changyu" uniqKey="Zheng C" first="Changyu" last="Zheng">Changyu Zheng</name>
<affiliation wicri:level="2"><nlm:affiliation>Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Hongchen" sort="Sun, Hongchen" uniqKey="Sun H" first="Hongchen" last="Sun">Hongchen Sun</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2016">2016</date>
<idno type="RBID">pubmed:26647730</idno>
<idno type="pmid">26647730</idno>
<idno type="doi">10.3892/mmr.2015.4608</idno>
<idno type="pmc">PMC4686068</idno>
<idno type="wicri:Area/Main/Corpus">000162</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000162</idno>
<idno type="wicri:Area/Main/Curation">000162</idno>
<idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000162</idno>
<idno type="wicri:Area/Main/Exploration">000162</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Effects of human vascular endothelial growth factor on reparative dentin formation.</title>
<author><name sortKey="Zhang, Juan" sort="Zhang, Juan" uniqKey="Zhang J" first="Juan" last="Zhang">Juan Zhang</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Xia" sort="Liu, Xia" uniqKey="Liu X" first="Xia" last="Liu">Xia Liu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yu, Weixian" sort="Yu, Weixian" uniqKey="Yu W" first="Weixian" last="Yu">Weixian Yu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Yingli" sort="Zhang, Yingli" uniqKey="Zhang Y" first="Yingli" last="Zhang">Yingli Zhang</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Shi, Ce" sort="Shi, Ce" uniqKey="Shi C" first="Ce" last="Shi">Ce Shi</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Ni, Shilei" sort="Ni, Shilei" uniqKey="Ni S" first="Shilei" last="Ni">Shilei Ni</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Liu, Qilin" sort="Liu, Qilin" uniqKey="Liu Q" first="Qilin" last="Liu">Qilin Liu</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Xiangwei" sort="Li, Xiangwei" uniqKey="Li X" first="Xiangwei" last="Li">Xiangwei Li</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Yingjian" sort="Sun, Yingjian" uniqKey="Sun Y" first="Yingjian" last="Sun">Yingjian Sun</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country>
<wicri:regionArea>Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130021</wicri:regionArea>
<wicri:noRegion>Jilin 130021</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Zheng, Changyu" sort="Zheng, Changyu" uniqKey="Zheng C" first="Changyu" last="Zheng">Changyu Zheng</name>
<affiliation wicri:level="2"><nlm:affiliation>Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.</nlm:affiliation>
<country xml:lang="fr">États-Unis</country>
<wicri:regionArea>Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892</wicri:regionArea>
<placeName><region type="state">Maryland</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Sun, Hongchen" sort="Sun, Hongchen" uniqKey="Sun H" first="Hongchen" last="Sun">Hongchen Sun</name>
<affiliation wicri:level="4"><nlm:affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</nlm:affiliation>
<orgName type="university">Université de Jilin</orgName>
<country>République populaire de Chine</country>
<placeName><settlement type="city">Changchun</settlement>
<region type="province">Jilin</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Molecular medicine reports</title>
<idno type="eISSN">1791-3004</idno>
<imprint><date when="2016" type="published">2016</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals (MeSH)</term>
<term>Calcification, Physiologic (drug effects)</term>
<term>Cell Differentiation (drug effects)</term>
<term>Child (MeSH)</term>
<term>Dental Pulp (cytology)</term>
<term>Dentin (metabolism)</term>
<term>Gene Expression Profiling (MeSH)</term>
<term>Gene Expression Regulation (drug effects)</term>
<term>Humans (MeSH)</term>
<term>Male (MeSH)</term>
<term>Rats, Wistar (MeSH)</term>
<term>Tooth, Deciduous (cytology)</term>
<term>Vascular Endothelial Growth Factor A (pharmacology)</term>
<term>Wound Healing (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes (MeSH)</term>
<term>Animaux (MeSH)</term>
<term>Calcification physiologique (effets des médicaments et des substances chimiques)</term>
<term>Cicatrisation de plaie (effets des médicaments et des substances chimiques)</term>
<term>Dent de lait (cytologie)</term>
<term>Dentine (métabolisme)</term>
<term>Différenciation cellulaire (effets des médicaments et des substances chimiques)</term>
<term>Enfant (MeSH)</term>
<term>Facteur de croissance endothéliale vasculaire de type A (pharmacologie)</term>
<term>Humains (MeSH)</term>
<term>Mâle (MeSH)</term>
<term>Pulpe dentaire (cytologie)</term>
<term>Rat Wistar (MeSH)</term>
<term>Régulation de l'expression des gènes (effets des médicaments et des substances chimiques)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Vascular Endothelial Growth Factor A</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Dent de lait</term>
<term>Pulpe dentaire</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Dental Pulp</term>
<term>Tooth, Deciduous</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Calcification, Physiologic</term>
<term>Cell Differentiation</term>
<term>Gene Expression Regulation</term>
<term>Wound Healing</term>
</keywords>
<keywords scheme="MESH" qualifier="effets des médicaments et des substances chimiques" xml:lang="fr"><term>Calcification physiologique</term>
<term>Cicatrisation de plaie</term>
<term>Différenciation cellulaire</term>
<term>Régulation de l'expression des gènes</term>
</keywords>
<keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Dentin</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Dentine</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Facteur de croissance endothéliale vasculaire de type A</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Child</term>
<term>Gene Expression Profiling</term>
<term>Humans</term>
<term>Male</term>
<term>Rats, Wistar</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Animaux</term>
<term>Enfant</term>
<term>Humains</term>
<term>Mâle</term>
<term>Rat Wistar</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">It is a challenge for dentists to save dental pulp in patients with pulp disease without resorting to root canal therapy. Formation of tertiary dentin to maintain pulp vitality is a key odontoblast response to dental pulp injury. Vascular endothelial growth factor (VEGF) is the most potent angiogenic and vasculogenic factor involved in tertiary dentin formation. It was hypothesized that VEGF may be used to treat pulp diseases such as pulpitis. To explore this hypothesis, the first step was to assess whether VEGF affects dental pulp cells to promote reparative dentin formation. In the current study, an AdCMV‑hVEGF vector was constructed to deliver hVEGF into dental pulp cells of exfoliated deciduous teeth (hDPCs) in vitro and dental pulp cells in a rat model in vivo. The collected data clearly demonstrated that hVEGF increased alkaline phosphatase and mineralization by enzymatic activity. RT‑qPCR data demonstrated that hVEGF significantly increased the expression levels of genes commonly involved in osteogenesis/odontogenesis. Data from the in vivo assays indicated that hVEGF enhanced pulp cell proliferation and neovascularization, and markedly increased formation of reparative dentin in dental pulp. The in vitro and in vivo data suggest that hVEGF may have potential clinical applications, thus may aid in the development of novel treatment strategies for dental pulpitis.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">26647730</PMID>
<DateCompleted><Year>2016</Year>
<Month>09</Month>
<Day>26</Day>
</DateCompleted>
<DateRevised><Year>2018</Year>
<Month>11</Month>
<Day>13</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1791-3004</ISSN>
<JournalIssue CitedMedium="Internet"><Volume>13</Volume>
<Issue>1</Issue>
<PubDate><Year>2016</Year>
<Month>Jan</Month>
</PubDate>
</JournalIssue>
<Title>Molecular medicine reports</Title>
<ISOAbbreviation>Mol Med Rep</ISOAbbreviation>
</Journal>
<ArticleTitle>Effects of human vascular endothelial growth factor on reparative dentin formation.</ArticleTitle>
<Pagination><MedlinePgn>705-12</MedlinePgn>
</Pagination>
<ELocationID EIdType="doi" ValidYN="Y">10.3892/mmr.2015.4608</ELocationID>
<Abstract><AbstractText>It is a challenge for dentists to save dental pulp in patients with pulp disease without resorting to root canal therapy. Formation of tertiary dentin to maintain pulp vitality is a key odontoblast response to dental pulp injury. Vascular endothelial growth factor (VEGF) is the most potent angiogenic and vasculogenic factor involved in tertiary dentin formation. It was hypothesized that VEGF may be used to treat pulp diseases such as pulpitis. To explore this hypothesis, the first step was to assess whether VEGF affects dental pulp cells to promote reparative dentin formation. In the current study, an AdCMV‑hVEGF vector was constructed to deliver hVEGF into dental pulp cells of exfoliated deciduous teeth (hDPCs) in vitro and dental pulp cells in a rat model in vivo. The collected data clearly demonstrated that hVEGF increased alkaline phosphatase and mineralization by enzymatic activity. RT‑qPCR data demonstrated that hVEGF significantly increased the expression levels of genes commonly involved in osteogenesis/odontogenesis. Data from the in vivo assays indicated that hVEGF enhanced pulp cell proliferation and neovascularization, and markedly increased formation of reparative dentin in dental pulp. The in vitro and in vivo data suggest that hVEGF may have potential clinical applications, thus may aid in the development of novel treatment strategies for dental pulpitis.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Juan</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Xia</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Yu</LastName>
<ForeName>Weixian</ForeName>
<Initials>W</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Yingli</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Shi</LastName>
<ForeName>Ce</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Ni</LastName>
<ForeName>Shilei</ForeName>
<Initials>S</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Qilin</ForeName>
<Initials>Q</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Li</LastName>
<ForeName>Xiangwei</ForeName>
<Initials>X</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Yingjian</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Department of Ophthalmology, The Second Hospital of Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Changyu</ForeName>
<Initials>C</Initials>
<AffiliationInfo><Affiliation>Molecular Physiology and Therapeutics Branch, National Institute of Dental and Craniofacial Research, National Institutes of Health, Bethesda, MD 20892, USA.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Hongchen</ForeName>
<Initials>H</Initials>
<AffiliationInfo><Affiliation>The Key Laboratory of Tooth Development and Bone Remodeling, Jilin Hospital of Stomatology, Jilin University, Changchun, Jilin 130021, P.R. China.</Affiliation>
</AffiliationInfo>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2015</Year>
<Month>11</Month>
<Day>23</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>Greece</Country>
<MedlineTA>Mol Med Rep</MedlineTA>
<NlmUniqueID>101475259</NlmUniqueID>
<ISSNLinking>1791-2997</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D042461">Vascular Endothelial Growth Factor A</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002113" MajorTopicYN="N">Calcification, Physiologic</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002454" MajorTopicYN="N">Cell Differentiation</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002648" MajorTopicYN="N">Child</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003782" MajorTopicYN="N">Dental Pulp</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D003804" MajorTopicYN="N">Dentin</DescriptorName>
<QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020869" MajorTopicYN="N">Gene Expression Profiling</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D005786" MajorTopicYN="N">Gene Expression Regulation</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008297" MajorTopicYN="N">Male</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017208" MajorTopicYN="N">Rats, Wistar</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014094" MajorTopicYN="N">Tooth, Deciduous</DescriptorName>
<QualifierName UI="Q000166" MajorTopicYN="N">cytology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D042461" MajorTopicYN="N">Vascular Endothelial Growth Factor A</DescriptorName>
<QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014945" MajorTopicYN="N">Wound Healing</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2015</Year>
<Month>01</Month>
<Day>20</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2015</Year>
<Month>11</Month>
<Day>05</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2015</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2015</Year>
<Month>12</Month>
<Day>10</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2016</Year>
<Month>9</Month>
<Day>27</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">26647730</ArticleId>
<ArticleId IdType="doi">10.3892/mmr.2015.4608</ArticleId>
<ArticleId IdType="pmc">PMC4686068</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Crit Rev Oral Biol Med. 1999;10(3):328-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10759412</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Oral Biol. 2000 Nov;45(11):1013-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11000388</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2000 Aug;79(8):1596-603</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11023281</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Quintessence Int. 2001 Oct;32(9):717-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11695140</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Endod. 2002 Jan;28(1):20-3</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11806643</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biomed Mater Res. 2002 Jun 15;60(4):668-78</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11948526</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2003 May 13;100(10):5807-12</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12716973</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biomaterials. 2004 Aug;25(18):4513-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15046942</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Oral Sci. 2004 Apr;112(2):163-70</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15056114</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>C R Seances Soc Biol Fil. 1993;187(5):582-95</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8069711</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 1996 Jan;81(1):26-30</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8850478</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Connect Tissue Res. 1996;35(1-4):267-72</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9084665</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Am J Physiol. 1998 Mar;274(3 Pt 2):H1054-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9530221</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 1998 Apr 17;273(16):9457-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9545272</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2004 Dec;83(12):896-902</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15557394</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Exp Physiol. 2005 May;90(3):315-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15778410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Pharm Res. 2005 Jul;22(7):1110-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16028011</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Oral Sci. 2006 Jun;114(3):216-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16776771</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Endod. 2006 Oct;32(10):951-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16982271</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Arch Oral Biol. 2008 Jan;53(1):9-13</Citation>
<ArticleIdList><ArticleId IdType="pubmed">17764655</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Angiogenesis. 2008;11(2):109-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18293091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Calcif Tissue Int. 2008 May;82(5):401-10</Citation>
<ArticleIdList><ArticleId IdType="pubmed">18488132</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2008 Dec;87(12):1144-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19029083</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Matrix Biol. 2009 May;28(4):221-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19348940</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Regen Med. 2009 Sep;4(5):697-707</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19761395</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2009 Sep;88(9):835-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">19767581</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2010 Mar;89(3):264-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20110511</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2010 Aug;89(8):791-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">20395410</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>PLoS One. 2011;6(4):e18721</Citation>
<ArticleIdList><ArticleId IdType="pubmed">21494568</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Eur J Oral Sci. 2013 Apr;121(2):92-100</Citation>
<ArticleIdList><ArticleId IdType="pubmed">23489898</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Dent Res. 2014 Aug;93(8):788-93</Citation>
<ArticleIdList><ArticleId IdType="pubmed">24928097</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
<li>États-Unis</li>
</country>
<region><li>Jilin</li>
<li>Maryland</li>
</region>
<settlement><li>Changchun</li>
</settlement>
<orgName><li>Université de Jilin</li>
</orgName>
</list>
<tree><country name="République populaire de Chine"><region name="Jilin"><name sortKey="Zhang, Juan" sort="Zhang, Juan" uniqKey="Zhang J" first="Juan" last="Zhang">Juan Zhang</name>
</region>
<name sortKey="Li, Xiangwei" sort="Li, Xiangwei" uniqKey="Li X" first="Xiangwei" last="Li">Xiangwei Li</name>
<name sortKey="Liu, Qilin" sort="Liu, Qilin" uniqKey="Liu Q" first="Qilin" last="Liu">Qilin Liu</name>
<name sortKey="Liu, Xia" sort="Liu, Xia" uniqKey="Liu X" first="Xia" last="Liu">Xia Liu</name>
<name sortKey="Ni, Shilei" sort="Ni, Shilei" uniqKey="Ni S" first="Shilei" last="Ni">Shilei Ni</name>
<name sortKey="Shi, Ce" sort="Shi, Ce" uniqKey="Shi C" first="Ce" last="Shi">Ce Shi</name>
<name sortKey="Sun, Hongchen" sort="Sun, Hongchen" uniqKey="Sun H" first="Hongchen" last="Sun">Hongchen Sun</name>
<name sortKey="Sun, Yingjian" sort="Sun, Yingjian" uniqKey="Sun Y" first="Yingjian" last="Sun">Yingjian Sun</name>
<name sortKey="Yu, Weixian" sort="Yu, Weixian" uniqKey="Yu W" first="Weixian" last="Yu">Weixian Yu</name>
<name sortKey="Zhang, Yingli" sort="Zhang, Yingli" uniqKey="Zhang Y" first="Yingli" last="Zhang">Yingli Zhang</name>
</country>
<country name="États-Unis"><region name="Maryland"><name sortKey="Zheng, Changyu" sort="Zheng, Changyu" uniqKey="Zheng C" first="Changyu" last="Zheng">Changyu Zheng</name>
</region>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SuicidDentistV1/Data/Main/Exploration

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000156 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000156 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SuicidDentistV1
   |flux=    Main
   |étape=   Exploration
   |type=    RBID
   |clé=     pubmed:26647730
   |texte=   Effects of human vascular endothelial growth factor on reparative dentin formation.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i   -Sk "pubmed:26647730" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd   \
       | NlmPubMed2Wicri -a SuicidDentistV1

This area was generated with Dilib version V0.6.39.
Data generation: Sun Oct 3 17:04:29 2021. Site generation: Sun Oct 3 17:05:17 2021

	Serveur d'exploration sur le suicide chez les dentistes
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration sur le suicide chez les dentistes

Effects of human vascular endothelial growth factor on reparative dentin formation.

Effects of human vascular endothelial growth factor on reparative dentin formation.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki